There are numerous manuals to guide practitioners in utilizing traditional additives in the construction of road, rail and dam construction but they fall short of specific guidance for non-standard additive-based ecofriendly and cost-effective soil stabilization. Increased attention has recently been on the use of non-standard additives for stabilizing weak soils due to environmental and cost concerns associated with traditional additives. We summarize the specific guidelines of using environmental-friendly enzymes to treat weak soils. We elaborate on the requirements and specifications for the Eko-Soil multi-enzyme product that is manufactured from water and proteins extracted from fermented exudes of plants. Specific tests (laboratory and field) and conditions required for soil stabilization using Eko-Soil enzyme are elaborated using the experience of past construction projects. The guide also elaborates enhancing the efficiency of enzymatic soil stabilization by correctly incorporating the required mixing proportions and pre-requisite condition tests. Professionals and practitioners will benefit from using novel eco-friendly sustainable stabilization techniques in the treatment of weak soils covering many applications including roads, foundations, water containment areas, landfills, working platforms and slope erosion control.