The benefits of e-government have attracted many countries across the world to introduce e-government. There is, however, lack of studies in validating a measurement model for evaluating the adoption of e-government in developing countries. The purpose of this study is to develop and validate a measurement model for evaluating the adoption of e-government in developing countries from the perspective of citizens. A pre-test, pilot study, reliability test, the convergent validity, the goodness of model fit and the discriminant validity are performed based on the data collected from 478 respondents using a survey instrument distributed in Saudi Arabia to validate a measurement model. This study, furthermore, validates the measurement model through the use of the structural equation modeling. The results reveal that a total of ten constructs with 39-items are valid for investigating the adoption of e-government in developing countries. This survey instrument provides a useful set of refined measures that can be used in similar contexts for investigating the adoption of e-government.