A nanoporous membrane based impedance sensing platform for DNAsensing with gold nanoparticle amplification

Nucleic acids based detection has become increasingly important for biomedical diagnostics, environ-mental monitoring and food screening. In this paper, a nanoporous alumina membrane based impedancesensing platform with gold nanoparticle tags amplification was developed for DNA sensing. Singlestranded DNA (ssDNA) probes were first covalently immobilized in nanopore walls via chemical link-ers. DNA hybridization in the nanopores induced blockage in the nanopores, which could be detected byelectrochemical impedance spectroscopy (EIS). The blockage signal could be further amplified by goldnanoparticles (AuNPs) conjugation and silver catalytic deposition method to reach a limit of detection(LOD) of 50 pM. This platform is promising for simple, rapid and sensitive DNA detection