Salmon (Oncorhynchus tshawytscha) is characterised by being rich in amino acids and long chain polyunsaturated fatty acids (PUFA) known for its health benefits. Pasta was prepared with different levels (5–20%) of salmon fish powder (SFP) and control made with semolina. Chemical composition, amino acid and fatty acid profiles of the pasta were determined. Fortification of pasta with SFP increased (p < 0.05) indispensable amino acid (IAA) content compared to the control. The incorporation of 5–20% SFP in the pasta formulation decreased the n6:n3 ratio from 19:1 (the control pasta) to 5:1 to 3:1, respectively. Digestible indispensable amino acid scores (DIAAS) were calculated using published data on amino acids digestibility to evaluate the protein quality of the pasta. IAA contents (mg IAA/g protein), were found to be highest in the enriched pasta with SFP (372–453) and lowest in the control pasta (328), all exceeded the FAO recommended daily allowance (277 mg IAA/g protein) and contributed on average 41% to total amino acid contents. The DIAAS value in the control pasta was 36% (lysine) and the pasta enriched with SFP containing had a DIAAS between 75 to 99%. Therefore, to fulfil the daily human AA requirement, the control pasta needs to be supplemented by amino acid and fatty acid rich salmon fish protein to achieve greater lysine concentration with DIAAS.