Textile substrates can be coated with suitable polymer solutions to enhance the surface functionality. This paper highlights the deposition of chitosan (CHT) on wool nonwoven substrates using traditional pad-dry-cure method; and its potential application in medical textiles specifically wound dressing products. Wool nonwoven substrates were prepared by pre-treating with BMIMCl (ionic liquid) and application with different concentrations of CHI. The liquid uptake and antimi crobial efficacy was evaluated. It was shown that 0.3% CHI treated samples demonstrated good liquid uptake and excellent antimicrobial properties.