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Complexation of plasmid DNA and poly(ethylene oxide)/poly(propylene oxide) polymers for safe gene delivery

journal contribution
posted on 2024-11-02, 07:53 authored by Hemant Daima, Shiv Shankar, Amanda Gammilonghi, Selvakannan PeriasamySelvakannan Periasamy, Suresh BhargavaSuresh Bhargava, Vipul BansalVipul Bansal
Gene delivery is the process of introducing foreign genetic material, such as DNA or RNA, into host cells. Gene therapy utilizes gene delivery to deliver genetic material with the goal of treating a disease or condition in the cell. Actual viral vectors may have side effects, while actual systems using metal nanoparticles for gene delivery are toxic. Therefore, we designed here a biocompatible tri-block copolymer PEO20-PPO69-PEO20as a gene delivery vector [PEO: poly(ethylene oxide); PPO: poly(propylene oxide)]. We studied the conjugation of PEO20-PPO69-PEO20and DNA using various techniques. Results of gel retardation assay along with zeta potential and dynamic light scattering provide evidence of DNA sequestration. Fourier transform infrared spectroscopy and X-ray photoelectron spectroscopy show that the PO43-groups of plasmid DNA are primarily involved during nanoconjugate construction. The integrity and functionality of plasmid DNA within the cellular environment is further demonstrated by the expression of green fluorescent protein gene in Escherichia coli. Overall, our findings support the use of block copolymers as delivery systems for mammalian and plant cells.

History

Journal

Environmental Chemistry Letters

Volume

16

Issue

4

Start page

1457

End page

1462

Total pages

6

Publisher

Springer

Place published

Germany

Language

English

Copyright

© 2018 Springer International Publishing AG, part of Springer Nature

Former Identifier

2006085707

Esploro creation date

2020-06-22

Fedora creation date

2019-04-30

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