This paper aims to improve the performance of the permanent magnet synchronous motor (PMSM) speed regulation system by combining a novel disturbance observer (DOB) with the super-Twisting sliding mode (STSM) technique. First, a STSM controller is constructed to eliminate the adverse effects of the lumped disturbance in the PMSM speed regulation system. A novel DOB is introduced to estimate and compensate the lumped unknown disturbance, which constitutes a composite controller with a feedforward compensation term and a state-feedback control. As a result, the gain of the composite sliding mode controller can be significantly reduced, which will improve the performance of the closed-loop PMSM speed regulation system. The validity and robustness of the proposed composite control scheme are fully verified by simulation and experimental results.