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Development of chorion-less zebrafish embryos in millifluidic living embryo arrays

journal contribution
posted on 2024-11-03, 15:18 authored by Nurul Hajar Mohd Fuad, Jan Kaslin, Donald WlodkowicDonald Wlodkowic
Dechorionation of zebrafish embryos has been proposed as a tool to improve toxicity bioassays because the chorion membrane forms a molecular barrier that can slow down or prevent chemicals from reaching the embryo. Despite its potential importance for experimental and predictive toxicology, the culture of dechorionated zebrafish embryos in a microfluidic environment has so far not received any attention. Here, we demonstrate a new Lab-on-a-Chip technology capable of automated, hydrodynamic immobilization of dechorionated embryos of Danio rerio. We show that chorion-less embryos can develop normally under microfluidic perfusion and be successfully used for on-chip developmental toxicity bioassays.

Funding

Ecotoxicology-on-a-chip: towards smart devices in environmental biomonitoring

Australian Research Council

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Safety of Lung Delivered Tissue Plasminogen Activator

United States Department of Health and Human Services

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History

Journal

Biomicrofluidics

Volume

11

Number

051101

Issue

5

Start page

1

End page

5

Total pages

5

Publisher

American Institute of Physics

Place published

United States

Language

English

Former Identifier

2006081365

Esploro creation date

2020-06-22