Groundwater CSI: Unravelling Pollution Sources in Complex Environments with Liquid Chromatography Triple Quadrupole Mass Spectrometry

Wastewater treatment plants (WWTP) are used worldwide to purify domestic and industrial wastewater before it is returned to the environment. Even after treatment, wastewater may still contain a variety of contaminants, including nutrients (usually nitrogen and phosphorus) and organic pollutants, such as endocrine disruptors and pesticides. These compounds can leak into groundwater via old and/or damaged infrastructure, leaching from biosolids storage areas and/or release of effluents. However, similar contaminant impacts can come from other sources, such as agriculture. It is often very difficult to distinguish the true source of such contamination, especially where wastewater treatment plants are located in agricultural areas. Robust and sensitive techniques are needed to characterize impacts where there are multiple potential sources. This study analyzed a variety of synthetic chemicals using liquid chromatography triple quadrupole mass spectrometry (LC–QQQ–MS) to generate unique fingerprints of pollution. These were used to distinguish the impact on the local groundwater of a WWTP in southeast Victoria (Australia) from that of local agriculture. The use of such novel tracers could become a valuable tool in environmental monitoring, management, and remediation in the future.