In ecology, multi-scale analyses are commonly performed to identify the scale at which a species interacts with its environment (intrinsic scale). This is typically carried out using multi-scale species environment models that compare the relationship between ecological attributes (e.g., species diversity) measured with point data to environmental data (e.g. vegetation cover) for the surrounding area within buffers of multiple sizes. The intrinsic scale is identified as the buffer size at which the highest correlation between environmental and ecological variables occurs. We present the first investigation of how the spatial resolution of remote sensing environmental data can influence the identification of the intrinsic scale using multi-scale species¿environment models. Using the virtual ecologist approach we tested this influence using vegetation cover spatial data and a simulated species environment relationship derived from the same spatial data.