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The nanoscience behind the art of: In-meso crystallization of membrane proteins

journal contribution
posted on 2024-11-02, 05:20 authored by Alexandru Zabara, Thomas Meikle, Janet Newman, T Peat, Charlotte ConnCharlotte Conn, Calum DrummondCalum Drummond
The structural changes occurring at the nanoscale level within the lipid bilayer and driving the in-meso formation of large well-diffracting membrane protein crystals have been uniquely characterized for a model membrane protein, intimin. Importantly, the order to order transitions taking place within the bilayer and the lipidic nanostructures required for crystal growth have been shown to be general, occurring for both the cubic and the sponge mesophase crystallization pathways. For the first time, a transient fluid lamellar phase has been observed and unambiguously assigned for both crystallization pathways, present at the earliest stages of protein crystallogenesis but no longer observed once the crystals surpass the size of the average lyotropic liquid crystalline domain. The reported time-resolved structural investigation provides a significantly improved and general understanding of the nanostructural changes taking place within the mesophase during in-meso crystallization which is a fundamental advance in the enabling area of membrane protein structural biology.

Funding

Biomimetic lipidic self-assembly materials for protein encapsulation

Australian Research Council

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History

Journal

Nanoscale

Volume

9

Issue

2

Start page

754

End page

763

Total pages

10

Publisher

Royal Society of Chemistry .

Place published

United Kingdom

Language

English

Copyright

© The Royal Society of Chemistry 2017.

Former Identifier

2006076374

Esploro creation date

2020-06-22

Fedora creation date

2017-08-10

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