The so-called clumping factor (Ω) quantifies deviation from a random 3D distribution of material in a vegetation
canopy and therefore characterises the spatial distribution of gaps within a canopy. Ω is essential to convert
effective Plant or Leaf Area Index into actual LAI or PAI, which has previously been shown to have a significant
impact on biophysical parameter retrieval using optical remote sensing techniques in forests, woodlands, and
savannas. Here, a simulation framework was applied to assess the performance of existing in situ clumping
retrieval methods in a 3D virtual forest canopy, which has a high degree of architectural realism. The virtual
canopy was reconstructed using empirical data from a Box Ironbark Eucalypt forest in Eastern Australia.
Hemispherical photography (HP) was assessed due to its ubiquity for indirect LAI and structure retrieval.
Angular clumping retrieval method performance was evaluated using a range of structural configurations based
on varying stem distribution and LAI. The CLX clumping retrieval method (Leblanc et al., 2005) with a segment
size of 15° was the best performing clumping method, matching the reference values to within 0.05 Ω on average
near zenith. Clumping error increased linearly with zenith angle to > 0.3 Ω (equivalent to a 30% PAI error) at
75° for all structural configurations. At larger zenith angles, PAI errors were found to be around 25–30% on
average when derived from the 55–60° zenith angle. Therefore, careful consideration of zenith angle range
utilised from HP is recommended. We suggest that plot or site clumping factors should be accompanied by the
zenith angle used to derive them from gap size and gap size distribution methods. Furthermore, larger errors and
biases were found for HPs captured within 1 m of unrepresentative large tree stems, so these situations should be
avoided in practice if possible.