Examining changes in sensitivity and functionality of mechanosensitive ion channel protein Piezo 1 exposed to low-level radiofrequency radiation

The worldwide increase in the use of wireless telecommunications devices, mainly mobile phones, has resulted in increased human exposure to radiofrequency (RF) radiation. RF exposure imparts heat on the body through energy deposition that may interact with other mechanosensitive pathways, such as through the mechanosensitive ion channels, which are essential for maintaining normal physiological processes such as balance and touch. This project is focused on studying the biological effects of low-power RF radiation on the expression and function of mechanosensitive ion channel protein (Piezo-1). To evaluate the effects of long-term exposures, cells were exposed at the frequency of 1800 MHz and power of 17 dBm for a period of 2 and 4 hrs using the transverse electromagnetic (TEM) cell exposure system. To study the effects of short-term RF radiation (10 min), exposures emitted from the mobile phone headset at the frequency of 845 MHz and powers of -15 dBm and -5 dBm were applied to cell cultures. The obtained results demonstrate that non-thermal exposures at specific frequencies and powers modulate the biological activity of studied ion channels. The findings of this study will assist in identifying the thresholds of microwave (MW) exposures affecting these selected proteins and be useful in providing information on appropriate exposure guidelines.